Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (M. musculus) | NOG (NOD/Shi-PrkdcscidIl2rγtm1Sug/Jic) mouse | Central Institute for Experimental Animals | Female and Male, 6–10 week-old | |
| Strain, strain background (M. musculus) | C57BL/6 mouse | CLEA Japan, Inc | Female, 6–8 week-old | |
| Cell line (Homo-sapiens) | G3Thi | Takara Bio Inc | TKR-6163 | Human kidney cell line derived packaging cell line |
| Cell line (Homo-sapiens) | TE14 | RIKEN BioResource Center | Human esophageal squamous cancer cell lines | |
| Cell line (Homo-sapiens) | LK2-hGPC1 | Previous paper (Harada et al., 2017), see Materials and methods | Human lung squamous cancer cell lines expressing hGPC1 | |
| Cell line (Homo-sapiens) | LK2-mock | Previous paper (Harada et al., 2017), see Materials and methods | Human lung squamous cancer cell lines expressing empty vector | |
| Cell line (M. musculus) | PG13 | Takara Bio Inc | Murine leukemia cell line | |
| Cell line (M. musculus) |
EL4 | National Cancer Institute, National Institutes of Health (MD, USA) | Mouse T cell lymphoma cell line | |
| Cell line (M. musculus) |
MCA205 | National Cancer Institute, National Institutes of Health (MD, USA) | Mouse sarcoma cell line | |
| Cell line (M. musculus) |
MCA205-mGPC1 | This paper, see Materials and methods | Mouse sarcoma cell line expressing mGPC1 | |
| Cell line (M. musculus) |
MC38-mGPC1 | This paper, see Materials and methods | Mouse colon adenocarcinoma cell line expressing mGPC1 | |
| Transfected construct (Gallus gallus domesticus, Homo-sapiens) | hCAR-LH | This paper, see Materials and methods and Figure 2—figure supplement 1 | ||
| Transfected construct (Gallus gallus domesticus, Homo-sapiens s) | hCAR-HL | This paper, see Materials and methods and Figure 2—figure supplement 1 | ||
| Transfected construct (Gallus gallus domesticus, M. musculus) |
mCAR | This paper, see Materials and methods and Figure 4—figure supplement 1 | ||
| Antibody | OKT-3 (anti-human CD3 mAb) | Thermo Fischer Scientific | Cat # 16-0037-81, RRID:AB_2619696 | T cell activation, 50 ng/ml |
| Antibody | Anti-mouse PD-1 Ab (clone: J43) | Bio X Cell | BE-0033–2, RRID:AB_1107747 | In vivo injection, 200 mg/mouse |
| Antibody | Anti-human IFNγ Ab (M700A) | Thermo Fisher Scientific | Cat # M700A, RRID:AB_223578 | For ELISA |
| Antibody | Anti-human IFNγ Ab (M700B) | Thermo Fisher Scientific | Cat # M700B | For ELISA |
| Antibody | anti-GPC1 Ab (clone: 1–12) | previous paper (Harada et al., 2017) | IHC-F(0.5 μg/ml) | |
| Antibody | anti-GFP mAb (clone: 1E4) | Medical and Biological Laboratories |
Code # M-048–3, RRID:AB_591823 | IHC-P(0.5 μg/ml) |
| Antibody | APC-donkey-anti-IgY Ab | Jackson immuno Research Inc | Code # 703-136-155, RRID:AB_2340360 | FACS (1:50) |
| Antibody | PE-donkey-anti-IgY Ab | Jackson immuno Research Inc | Code # 703-116-155, RRID:AB_2340358 | FACS (1:50) |
| Antibody | V500- anti-mCD45 mAb | BD Biosciences | Cat # 561487, RRID:AB_10697046 | FACS (1:50) |
| Antibody | BV421-anti-mCD3 mAb | Biolegend | Cat # 562600, RRID:AB_11153670 | FACS (1:50) |
| Antibody | Alexa Fluor 700-anti-mCD8 mAb | BD Biosciences | Cat # 557959, RRID:AB_396959 | FACS (1:50) |
| Sequence-based reagent | human GPC-1 (Hs00892476_m1) | Applied Biosystems | Cat # 4331182 | |
| Sequence-based reagent | mouse GPC-1 (Mm00497305_m1) | Applied Biosystems | Cat # 4331182 | |
| Peptide, recombinant protein |
Human GPC1 |
Biolegend | Cat # 757206 | |
| Peptide, recombinant protein | Mouse GPC1 |
Biolegend | Cat # 757306 | |
| Peptide, recombinant protein | MuLV gp70 p15E | Medical and Biological Laboratories | Code # TS-M507-P | KSPWFTTL |
| Peptide, recombinant protein | H-2Kb-restricted b-galactosidase | Medical and Biological Laboratories | Code # TS-M501-P | DAPIYTNV |
| Commercial assay or kit | Series S Sensor Chip CM5 | GE Healthcare Life Sciences | Cat # BR-1005–30 | |
| Commercial assay or kit | Mouse Antibody Capture Kit |
GE Healthcare Life Sciences | Cat # BR1008-38 | |
| Commercial assay or kit | Mouse IFNγ ELISA set | BD Biosciences | Cat # 555138 | |
| Software, algorithm | Kaluza 1.2 | BECKMAN COULTER | ||
| Software, algorithm | GraphPad Prism 7.0 | GraphPad software | ||
| Other | FDA Standard Frozen Tissue Array-Human Adult Normal | BioChain Institute Inc | Cat # T6234701-1 | |
| Other | human normal cervix tissues and cervical carcinoma tissues | This paper, see Materials and methods | ||
| Other | Cr51 | Japan Radioisotope Association |