Table 2. Rescue experiments with heat shock fn1a-mKikGR at the 10–12 somite stage on pre-sorted fn1a-/-; fn1b-/- embryos.
For each experiment, fn1a-/+; fn1b-/+;hsp70: fn1a-mKikGR adults were crossed and embryos sorted for the fn1a-/-; fn1b-/- morphological phenotype were heat shocked at the 10–12 stage and assayed for somite border defects at 24 hpf. 1 or two embryos per experiment were not heat shocked as a control. Four experiments were performed and the numbers shown in the table represent the total number of embryos with each phenotype. * HS at the 12-somite stage will rescue body elongation and head development defects observed in the fn1a-/-; fn1b-/- embryos, however heat shock will not fully rescue border defects of the first 1–6 somites.
| Embryos with no heat shock | Heat shocked embryos | ||
|---|---|---|---|
| Fluorescent | Non-fluorescent | ||
| Rescued phenotype * | - | 13 | 0 |
| fn1a-/-; fn1b-/- | 5 | 0 | 1 |