Figure 2. Impaired thymocyte development and peripheral T cell compartment in T-PRMT5^Δ/Δ mice.

(A) Gating strategy for analysis of thymocyte populations and representative thymic Treg percentage plots. Live/dead gating was also performed after FSC/SSC but is not displayed. (B–G) Thymocytes were analyzed by flow cytometry and (B) B220^–, (C) DN, (D) DP, (E) CD4 SP, (F) Treg, and (G) CD8 SP thymocyte numbers were calculated. (H–N) Splenocytes were analyzed by flow cytometry (gating strategy in I and H) B220^– splenocyte, (J) CD4⁺, (K) Treg, (L) CD8⁺, (M) CD4⁺ Tem, Tcm, and naive populations, and (N) CD8⁺ Tem, Tcm, and naive populations were calculated. (O) iNK T cell flow cytometric analysis gating strategy and total (P) thymic and (Q) splenic iNKT cell numbers. Data are representative of 4 independent experiments. Shown n = 4–5 independent mice of matched age. One-way ANOVA, followed by Sidak’s (B–H and J–N) or Tukey’s (P and Q) multiple-comparisons test: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Bar graphs display mean ± SD. DN, double negative; DP, double positive; SP, single positive; Tem, effector memory T cells; Tcm, central memory T cells.