Figure 4. Prmt5 deficiency in iCD4-PRMT5^Δ/Δ T cells abrogates Th17 cell differentiation.

(A) Experimental design for Th cell differentiation in iCD4-PRMT5^Δ/Δ mice. Naive CD4⁺ T cells isolated from iCD4-PRMT5^Δ/Δ mice were polarized into (B–H) Th1, (I–O) Th2, (P–V) Th17, or (W–Z) Tregs and assessed by flow cytometry. Cells shown are gated on live (LiveDead Dye^–) CD44⁺ cells. Th1 cells were assessed by T-bet⁺IFN-γ⁺ cell (C) percentage and (F) number, IFN-γ⁺ cell (D) percentage and (G) number, T-bet⁺ (E) cell percentage, and (H) mean fluorescence intensity (MFI) by flow cytometry. Th2 cells were assessed by GATA-3⁺IL-4⁺ cell (J) percentage and (M) number, IL-4⁺ cell (K) percentage and (N) number, GATA-3⁺ (L) cell percentage, and (O) MFI by flow cytometry. Th17 cells were assessed by RORγt⁺IL-17⁺ cell (Q) percentage and (T) number, IL-17⁺ cell (R) percentage and (U) number, RORγt⁺ (S) cell percentage, and (V) MFI by flow cytometry. Tregs were assessed by Foxp3⁺CD25⁺ (X) cell percentage and (Y) number, and (Z) Foxp3 MFI. Data pooled from 3 independent experiments including n = 6–12/group. For Th2 cells, data from 2 independent experiments, n = 2–5/group. One-way ANOVA, followed by Tukey’s multiple-comparisons test was used. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Graphs are box-and-whisker plots (box extends from 25th to 75th percentiles, all points shown, whiskers extend from min to max, line represents median).