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. 2020 Mar 3;130(4):1767–1781. doi: 10.1172/JCI133473

Figure 6. iPTH and butyrate fail to improve trabecular bone structure, stimulate bone turnover, expand Tregs, and induce Wn10b expression in 12-week-old GPR43–/– mice.

Figure 6

(A and B) μCT scanning measurements of bone volume fraction (BV/TV) and trabecular thickness (Tb.Th) (n = 10 mice/group). (C and D) Serum osteocalcin and CTX levels (n = 10 mice/group). (E–G) PP and BM Tregs (TCR-β+CD4+Foxp3+ cells) (n = 10 mice/group). (H and I) Wnt10b mRNA levels in whole BM cells and sorted BM CD8+ T cells (n = 5 mice/group). In A–I, GPR43–/– mice and WT littermates (GPR43+/+ mice) were treated with iPTH or vehicle for 4 weeks. Mice were sacrificed and analyzed at 12 weeks of age. (J–L) BV/TV, Tb.Th, and serum osteocalcin in mice treated with vehicle or iPTH, and butyrate (But) for 4 weeks starting at 8 weeks of age. Mice were also treated with antibiotics (Abx) for 6 weeks, starting at 6 weeks of age. Mice were sacrificed and analyzed at 12 weeks of age (n = 5 mice/group). Data were expressed as mean ± SEM. All data were normally distributed according to the Shapiro-Wilk normality test. All data were analyzed by 2-way ANOVA and post hoc tests, applying Bonferroni’s correction for multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 compared with the indicated group in the post hoc tests.