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. 2020 Mar 16;130(4):2129–2145. doi: 10.1172/JCI132691

Figure 9. Depleting ESRP2 exacerbates alcohol-induced steatohepatitis in mice.

Figure 9

(A) The serum levels of ALT in WT and Esrp2-KO mice treated with alcohol as per the Gao binge model. Results are graphed as dot plots (WT pair: white circles with white bar; WT 10d + 1B, white squares with white bar; Esrp2-KO pair, black circles with gray bar; Esrp2-KO 10d + 1B, black squares with gray bar) showing mean ± SEM (red bars) (n = 3–7 individuals/group). (B) Hepatic TG content in WT and Esrp2-KO mice with or without alcohol exposure. (C) H&E and ORO staining in liver sections of these mice. Yellow bracket indicates ductular reaction. Scale bars: 20 μm. (D–F) qRT-PCR analysis for Pparγ (D), inflammatory cytokines (Tnfα and Il1β) and immune cell markers (Cd68 and Ly6G) (E), and fibrogenic markers (Col1α1 and Snail) (F) in WT and Esrp2-KO mice exposed to alcohol or pair-fed control diet. All results are graphed as dot plots (WT pair, white circles; WT 10d + 1B, white squares; Esrp2-KO pair, black circles; Esrp2-KO 10d + 1B, black squares) showing mean ± SEM (red bars) (n = 3–7 individuals/group). All statistical analyses were performed by 2-way ANOVA with Tukey’s corrections.