Figure 5. Lipolysis is required for epinephrine-induced increases in body temperature resulting from β-adrenergic activity in BAT.

(A) BAT long-chain acyl-CoA concentrations in 48-hour-fasted rats infused with epinephrine ± atglistatin ± Liposyn. (B) Body temperature prior to refeeding. (C) Food intake within 2 hours of refeeding. In A–C, *P < 0.05, **P < 0.01, ****P < 0.0001 by ANOVA with Bonferroni’s multiple-comparisons test. (D) WAT and BAT β₃-adrenergic receptor expression in Adrβ₃ BAT-specific KO mice. Full, uncut gels are shown in Supplemental Figure 15. (E) Plasma leptin concentrations 60 minutes after dextrose gavage. (F) Body temperature. In D–F, *P < 0.05, ****P < 0.0001 by 2-tailed unpaired Student’s t test. n = 5 (WT) and n = 11 (Adrβ₃ BKO). In all panels, data are presented as mean ± SEM. If no symbol appears, groups are not statistically different.