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. 2020 Mar 16;130(4):2054–2068. doi: 10.1172/JCI125898

Figure 4. Neogenin induction in degenerating Rd1 rods, Rd1 cones, and human photoreceptors.

Figure 4

(A) Representative immunostained cryosections of P12 C57BL/6J mouse retina or Rd1 mouse retina stained for neogenin (red), rhodopsin (green), and nuclei (DAPI; blue). In C57BL/6J retina, neogenin expression is largely restricted to the outer limiting membrane, with rhodopsin localized predominantly in the outer segments. In Rd1 mice, rhodopsin is misexpressed in the cell bodies of rods and is coexpressed in the same cells with neogenin (arrow). Scale bar: 20 μm. (B) Representative images of P16 C57BL/6J retina and P16 Rd1 retina. At this stage, cone degeneration has commenced in Rd1 mice, and the increased neogenin (green) expression in photoreceptors is also observed in cells staining positive for a marker of cones (Cone Arrestin; red). Arrows indicate examples of colabeling. Dashed lines indicate the borders of the ONL. Note the reduction in the size of the ONL at this age in Rd1 mice due to the majority of rods being gone. Scale bar: 20 μm. (C) Representative immunostained cryosections of retinas of a postmortem 54-year-old human donor not diagnosed with RP (Adult with no RP), a 63-year-old adult diagnosed with RP (Adult with RP #1), a 64-year-old adult donor diagnosed with RP (Adult with RP #2), and a 66-year-old adult donor diagnosed with RP (Adult with RP #3). Sections were stained for neogenin (red), rhodopsin (green), and nuclei (DAPI; blue). Note the presence of neogenin in the IS/OS in the diseased retinas. Arrow indicates expression of neogenin and misexpressed rhodopsin in the same cell. Scale bars: 20 μm.