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. 2020 Mar 16;130(4):2041–2053. doi: 10.1172/JCI129635

Figure 4. Characterization of TRPC6 in platelets.

Figure 4

(A, B) mRNA expression of TRPC isoforms TRPC1 and TRPC6 in platelets from WT, CFTR–/–, CFfl/fl, CF-LysM, and CF-PF4 mice. TRPC isoforms 2, 3, 4, 5, and 7 were undetectable (not shown). (C) Immunofluorescence staining and (D) flow cytometry analysis of CD41 (red) and TRPC6 (blue) in platelets from WT and TRPC6–/– mice (representative of 3 independent experiments). Scale bar: 2.5 μm. (E and F) CD62P expression on platelets from (E) WT, CFTR–/–, TRPC6–/–, and CFTR–/– × TRPC6–/– mice, and (F) CFfl/fl and CF-PF4 mice after thrombin challenge with or without incubation with vehicles, CF172, or CF172 plus SKF-96365 (SK). Data are mean ± SEM of 5 to 11 animals per group. Data were analyzed by 2-way ANOVA. *P ≤ 0.05; **P ≤ 0.01; ****P ≤ 0.0001.