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. Author manuscript; available in PMC: 2021 Jul 15.
Published in final edited form as: Biol Psychiatry. 2019 Oct 1;88(2):139–149. doi: 10.1016/j.biopsych.2019.09.018

Figure 3.

Figure 3.

Parameters affecting excitability and an overexpression of Kcnc1 and Kcnc2 in LR CA3 neurons. A.CA3 excitability (encoded in the radius of the dot, see Methods) as a function of the amplitude of the fast potassium currents (y-axis) and the kinetics or decay time constant of the fast potassium current (x-axis). The larger dots are generally observed in the neurons with fast kinetics or fast decay time of the fast potassium currents. B. DG excitability (encoded in the radius of the dot, see Methods) as a function of the amplitude of the fast potassium currents (y-axis) and the kinetics or decay time constant of the fast potassium current (x-axis). The larger dots are observed in the neurons with fast kinetics or fast decay time and higher amplitudes of the fast potassium currents. C-E. The excitability in all the groups correlates with sodium currents (at −20 mV). This correlation is much stronger in NR neurons compared to control and LR neurons. F-H. A significant correlation is observed between excitability and the amplitude of the fast potassium currents at 0 mV in LR and NR neurons. The excitability in control neurons does not correlate with the amplitude of the fast potassium currents at 0 mV. I. Blocking of different potassium channels reduced excitability of CA3 BD neurons but increased excitability of CA3 control neurons; Dendrotoxin (DTX) at 200 nM significantly increased excitability of control neurons. 1 mM Tetraethylammonium (TEA) most drastically reduced hyperexcitability of CA3 LR neurons, but 0.3 mM of 4-aminopyridine (4-AP) and DTX also significantly reduced their hyperexcitability. 4-AP reduced excitability of CA3 NR neurons. A total number of 53 control, 28 control TEA, 27 control 4-AP, 28 control DTX, 47 LR, 39 LR TEA, 18 LR 4-AP, 29 LR DTX, 104 NR, 28 NR TEA, 28 NR 4-AP and 28 NR DTX neurons were recorded. J. qPCR results (fold change) of Kcnc1 showed that CA3 LR neurons have elevated levels compared to control neurons. K. Fold change values of Kcnc2 showed a decreased expression in NR neurons and an increased expression in LR neurons compared to CA3 control neurons. Asterisks represent statistical significance by the following code: * p value<0.05, **p value<0.01, ***p<0.001, ****p<0.0001. Error bars represent standard error.