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. 2020 Feb 4;122(7):986–994. doi: 10.1038/s41416-020-0744-7

Fig. 4. Nintedanib suppresses the cytokine secretion of CAFs.

Fig. 4

a Cytokine array comparing CAF–CM and nintedanib-treated CAF–CM. IL-6, IL-8, VEGF, VCAM1 and osteopontin were reduced by nintedanib treatment. The right panel shows the density ratio of array dots between CAF–CM and nintedanib-treated CAF–CM. Cytokine array was performed using CAF1. b ELISA of IL-6 and IL-8 in CAF–CM and nintedanib-treated CAF–CM. IL-6 and IL-8 were reduced by nintedanib treatment (n = 5). *P < 0.01. c The effects of CAF–CM and nintedanib-treated CAF–CM on the phosphorylation of STAT3 in HuCCT1 and RBE cell lines were evaluated by Western blotting. CAF–CM increased the phosphorylation of STAT3 in ICC cell lines, but the increasing effects were eliminated by nintedanib treatment.