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. 2020 Apr 1;7:50. doi: 10.1038/s41438-020-0273-9

Fig. 3. MdbHLH3 transcriptionally activates and positively regulates MdDEP1 gene expression.

Fig. 3

a The effector and reporter constructs in the binary vectors were introduced into apple calli for the GUS activity assays. b PMdDEP1::uidA transgenic apple calli with or without the 35S::MdbHLH3 effector were grown at 25 °C in the dark and stained to visualize the GUS activity. c GUS activity in the transgenic apple calli as labeled. d Relative transcripts of MdbHLH3 and MdDEP1 in transgenic apple lines overexpressing MdbHLH3 (MdbHLH3-27, MdbHLH3-36, MdbHLH3-44) and the WT control. The data represent the means ± SE of three independent experiments. Statistical significance was determined using Student’s t-test; *P < 0.01 and **P < 0.001