Figure 2.

Co-expression of TRAM in AdHu5-TIPEGFP vector impacts on the transgene-specific immune responses. Groups of BALB/c mice were immunized i.m. at day 0 with AdHu5-TIPEGFP (SE) (black dots, black lines in all graphs) or with AdHu5-TIPEGFP-tram (SE) vectors (open circles, dotted lines in all graphs), at the doses indicated (a,b), or at 4 × 10⁸ iu/mouse (c–e). Specific CD8⁺ T cell responses to Pb9 peptide (a,c) or CD4+ responses to P15 peptide (b,d) were measured by IFN-γ ELIspot at week 2. Antibody titres against GFP protein were measured at the same time point (e) and the long dotted lines represent the detection limit of the assay. Each dot represents one mouse and the short lines represent the geometric means per group. P values are indicated (2-way ANOVA).