Figure 3.

Effect of murine and human TRAM in mice by co-injection. Expression of murine TRAM impacts on the CD8+ immune response elicited by a co-injected vector. (a,b) Groups of C57BL/6 mice were immunized i.m. at day 0 with 10⁸vp AdHu5-Pfm128 alone (black dots, referred as Nil in the X axis) or mixed with AdHu5-TIPEGFP (black dots, referred as TIP in the X axis) or with AdHu5-TIPEGFP-tram vectors (open dots, referred as TIP-TRAM), at 10⁷ iu/mouse. Specific CD8⁺ T cell responses (a) and CD4⁺ responses (b) were measured by IFN-γ ELIspot at week 2. Each dot represents one mouse and the short lines represent the geometric means per group. Effect of human TRAM-encoding ChAd63 in mouse by co-injection (c,d). Groups of mice were immunized i.m. at day 0 with ChAd63-ME TRAP alone or was mixed with ChAd63-hTRAM, expressing human TRAM, at a 1:1 or 1:25 ratio. (c,d) A control group included ChAd63-ME TRAP mixed with similar ratios of an adenovirus encoding an irrelevant antigen (secreted alkaline phosphatase, ChAd63-SEAP). Specific CD8⁺ T cell responses (c) and CD4⁺ responses (d) were measured by IFN-γ ELIspot at week 2. Each dot represents one mouse and the short lines represent the geometric means per group. Only the group that received 25x TRAM had a response significantly superior to the group that received ChAd63-ME TRAP with no adjuvant (nil).