Fig. 4.

Overexpression of serpinB9 (Sb9) and surface CD107a reduces NK cell-mediated killing and decelerates their detachment from target cells. K562 target cells were transfected with Sb9, surface CD107a, or empty vector. a A 4 h ⁵¹Cr release assay in NK cells with the indicated K562 target cells. b Conjugate formation between NK cells with the indicated K562 target cells. The left panels show one representative experiment, and the quantification of three independent experiments is shown in the right panels. c The detachment from target cells was analyzed in a detachment assay. The left panel shows the results of one representative experiment, and the right panel shows the quantification of the half-life (n = 4–11). Values are shown as medians with interquartile ranges (*p < 0.05, ***p < 0.001, ns = not significant)