Fig. 6.

K562 cells lose activating NK cell ligands and adhesion molecules after NK cell-mediated killing. NK cells and K562 target cells were coincubated for 2 h and stained with the live/dead dye Zombie Aqua and Annexin V to distinguish live, early apoptotic and dead target cells (see Fig. S4). Cells were stained with antibodies against the indicated a ligands for activating NK cell receptors and b adhesion proteins. Expression levels of the indicated proteins were analyzed by subtracting the median fluorescence of the respective control stained sample from that of the specifically stained sample. Left panels show histograms from one representative experiment. Right panels show the quantification of independent experiments with NK cells from donors (n = 4, except CD58 and CD59 n = 3 and 41BBL n = 2). Values are shown as the mean ± SD (*p < 0.05, **p < 0.01 Friedman test with Dunn’s multiple comparison)