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. 2020 Mar 31;10:5718. doi: 10.1038/s41598-020-62749-7

Table 1.

Analytical HPLC conditions for Curcuma longa extract.

Parameters Conditions
Column Zorbax extended-C18 (C18, 4.6 mm × 150 mm, 5 µm)
Flow rate 0.8 mL/min
Injection volume 10 μL
UV detection 450 nm
Run time 30 min
Gradient Time (min) % A1 % B2
0 20 80
11 20 80
25 100 0
27 20 80
30 20 80

1 Acetonitrile;2 0.2% phosphoric acid (pH 3.5).