Skip to main content
. 2020 Feb 18;122(7):1037–1049. doi: 10.1038/s41416-020-0758-1

Fig. 1. Identification of the KLF5-targeting miRNA miR-4711-5p.

Fig. 1

a Candidate miRNAs were screened by assessing cell viability at 72 h after transfection in CRC cell lines. In three cell lines, miR-4711-5p remarkably suppressed cell viability. Parent cells were used as the control for normalisation. b Schematic illustration showing that KLF5 possesses three putative miR-4711-5p binding sites in its 3ʹ-UTR (WT). Four nucleotides in insert 1 and two nucleotides in insert 2 were deleted in the KLF5-mutant plasmids (MT). c In CRC cell lines, miR-4711-5p transfection significantly suppressed the luciferase activities of the two reporter plasmids containing three putative miR-4711-5p binding sites in the wild KLF5 3’-UTR (P < 0.01) but did not suppress those of KLF5-mutant reporter plasmids. d, e Real-time quantitative PCR and western blot analyses revealed that miR-4711-5p suppressed KLF5 expression at the mRNA and protein levels (P < 0.01). All data represent the mean ± SD.