Table 2.
Details of the genetic variants analyses, primer sequences and restriction enzymes used.
| Function | Gene | SNP ID | Alleles wt/v | Primer sequences 5′→3′ | Restriction enzyme |
|---|---|---|---|---|---|
| Transporters | ABCA1 | rs4149339 | C/T |
F: TCA GTC ATG ACT AGT GCC TA R: ATC CAA TAT CTG CAA AGC CA |
SspI |
| ABCC4 | rs9516521 | C/T |
F: AACCAAAAGGCTTACAGTCA R: CCTGAAGGCTTCTTGTTAGA |
HaeIII | |
| ABCC1 | rs212090 | A/T |
F: TCT ACC AGT TCT CGT TTT GG R: AAG CTT CAT AAC CAT GAG CA |
MboI | |
| rs212091 | A/G |
F: GATTCCACTTTGGGCTCTAA R: TTTTAAGTACTGTTCCGGGG |
DdeI | ||
| rs129081 | C/G |
F: GCAAGTCTTTGAGATGCTTC R: TTGCTCACTCTCAGTCTCTA |
MlyI | ||
| rs3743527 | C/T |
F: GACTTCTGGAGGAATTGGTT R: AGTTCCAGGTCCAGGTTAG |
BseRI | ||
| ABCB1 | rs17064 | A/T |
F: TTTTCAATGGTCAGTGTCCA R: GGTTCTGTAAGACCCAATGT |
ApoI | |
| ABCC5 | rs3805114 | A/C |
F: TTAGCATGTTTGCTGAACAC R: ATTTTCAATGGGTGAATCTG |
MnlI | |
| SLC22A16 | rs7756222 | C/T |
F: AGG GTT TCT GGA AGC TTT AG R: TGC CCC ATA AAG TAG CAA TT |
HhaI | |
| rs9487402 | T/G |
F: AGG GTT TCT GGA AGC TTT AG R: TGC CCC ATA AAG TAG CAA TT |
AluI | ||
| RALBP1 | rs12680 | C/G |
F: GGA GAT TGA GTA CTC TGC AG R: TCC CCA ATG CTA AAT GTA CC |
AvaII | |
| Drugs metabolizers | AKR1C3 | rs3209896 | A/G |
F: CTC CCC TAG TAA TGG AGT CA R: CAG GCA AAT CAC ACA GTT TT |
MnlI |
| ALDH5A1 | rs1054899 | A/C |
F: TCA AGT ATG TGT GTT ACG GG R: ACG GAC AAT TCT GTG CTA TT |
MboI | |
| CBR1 | rs20572 | C/T |
F: GTG GTG AAC GTA TCT AGC AT R: TGC ATC AGA GGA AAT CAC AA |
BfaI | |
| CYP1A2 | rs17861162 | C/G |
F: CCATGTTGGCTAGACTAGTC R: GGGTCAAACAACATGAAAGG |
BglI | |
| CYP2E1 | rs2480256 | C/T |
F: GCACTCCATCCTGGTCAACA R: ACGAGTGTGTGCTGGAGAAG |
TagI | |
| CYP1B1 | rs162562 | A/C |
F: CTGCTTCTCAATTAGCGTTT R: GAAAAGGGAATTTCTGGTCT |
MboII | |
| NOS3 | rs2566508 | G/T |
F: CTA GAC TCA AGC AAT CCT CC R: GGC TGT AGG TTA TAT GGG TG |
DdeI | |
| SULT4A1 | rs138057 | A/G |
F: CAGACTTCCCTCAGCACAT R: AGTGGTGAGAACAGAGGATG |
MnlI | |
| UGT2B15 | rs3100 | T/C |
F: GATCCAGTACCACTCTTTGG R: GCATCCAGTAACTCGTCATT |
MmeI | |
| UGT2B4 | rs1131878 | A/G |
F: ATTGTTCCAATTCACAGGTT R: AACCAAAAACCAGTTGTCAC |
AluI | |
| DPYD | rs291592 | A/G |
F: TGT GAC AGT TTC CAA ATT GC R: CAA ACT GAT TTG GCA CAC TT |
HpyCH4III | |
| rs291593 | C/T |
F: TGT GAC AGT TTC CAA ATT GC R: CAA ACT GAT TTG GCA CAC TT |
NdeI | ||
| GSTM3 | rs3814309 | C/T |
F: GAGACCTTCCCTCTCTATGT R: CTCATGCATTGCTTGTGTAG |
RsaI | |
| Dna Repair | ERCC1 | rs1046282 | A/G |
F: CAGGATTAATGGTCGTGGAT R: GTTAAGACCAATGGACCGAT |
HaeIII |
| rs3212986 | G/T |
F: CAG CTC CTT TAA TGA CTG GT R: AAG AAG CAG AGT CAG GAA AG |
MboII | ||
| ERCC4 | rs2276464 | G/C |
F: TTGTCTACAAAGGAGCCTTC R: CTGGACTTAGGGATCTCTGA |
SpeI | |
| rs2276466 | C/G |
F: TTCATGGAGCCTTCCTACTA R: CACAGGATGGTGAGAAATGA |
MnlI | ||
| rs4781563 | A/G |
F: ATCATGGTGCCTCTTTTGAT R: CCAAATGAGTTTTCTGAGCC |
DdeI/HaeIII | ||
| Nuclear receptors | NR1/2 | rs3732359 | A/G |
F: CAGTCTGTAGGGAGTGAAGC R: GATGCAGAGACACAGAATGA |
FokI |
| PGR | rs1824125 | G/T |
F: AACTTGGCGCTTAATAATCT R: AGGTGAGATTCAGACAAGTA |
AvaII | |
| rs561610 | C/T |
F: TCC AAA ACC CTT CTG GAA AA R: AGA CTG AAG AAG AAA GCC AG |
MboI | ||
| rs11224560 | C/T |
F: CTG GCT TTC TTC TTC AGT CT R: TAT TGG AGC ACC TAA GAG GA |
NlaIII |
The genotyping methods for polymorphisms in all genes were developed for this study; SNP-single nucleotide polymorphism; wt-wild type; v-SNP variation.