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. 2020 Mar 31;11(3):211. doi: 10.1038/s41419-020-2404-5

Fig. 4. Downregulation of CTR1 attenuates renal fibrosis.

Fig. 4

a Western blotting showing the expression of activated LOX, Elastin, Col3a and CTR1 in the kidneys after UUO or sham operation with delivery of the CTR1 shRNA–expressing plasmid or empty plasmid (n = 8). b The contents of insoluble and soluble collagen were determined with the microwave-assisted acid digestion method and the proportion of insoluble to soluble collagen were assessed (n = 8). c Representative micrographs of col3a immunostaining and Masson’s staining of kidney sections (n = 8). Original magnification, ×200. Bar = 50 μm. d CTR1 knockdown in NRK-49F cells was performed with a lentiviral shRNAmir system. LOX activity as determined using the Fluorometric Lysyl Oxidase Assay Kit in the culture medium of NRK-49F cells (n = 5). e Western blotting showing the expression of activated LOX, Elastin and Col3a in NRK-49F cells after CTR1 downregulation (n = 5). f The insoluble collagen, soluble collagen and the ratio of insoluble to soluble collagen were tested in NRK-49F cells (n = 3). g Immunofluorescence showing Col3a expression (n = 3). Original magnification, ×200. Bar = 50 μm. Data represent the mean ± SEM. *P < 0.05, **P < 0.01 versus sham-operated rats. #P < 0.05 versus the scramble-treated UUO kidneys. $P < 0.05, $$P < 0.01, $$$P < 0.001 versus the nontreated group. &P < 0.05, &&P < 0.01 versus with TGFβ1-treated cells.