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. 2020 Mar 25;11:355. doi: 10.3389/fimmu.2020.00355

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Copyright © 2020 Murugaiah, Varghese, Saleh, Tsolaki, Alrokayan, Khan, Collison, Sim, Nal, Al-Mohanna and Kishore.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Factor H and VCP inhibit transduction of MDCK cells by H1N1-pseudotyped lentiviral particles. Western blotting to show expression of hemagglutinin (HA) in purified H1N1 and H3N2 pseudotyped lentiviral particles (A). HA expression was evident at ~70 kDa. Luciferase reporter activity of H1N1 and H3N2 pseudotyped lentiviral particles-transduced cells with factor H and VCP (B). Significance was determined using the unpaired one-way ANOVA test (**p < 0.01 and ***p < 0.001) (n = 3).