Skip to main content
. 2020 Mar 25;8:175. doi: 10.3389/fcell.2020.00175

FIGURE 2.

FIGURE 2

3,4,5-Tri-O-caffeoylquinic acid (TCQA) stimulated the melanogenesis in human epidermal melanocyte (HEM). (A) Melanin content determination after 48, 72, and 96 h of treatment with various concentrations of TCQA and 200 nM of α-MSH used as positive control. (B) Cell viability determination. (C) Determination of the protein expression of the melanogenesis enzymes – TYR, TYRP1, and DCT – after 24 and 48 h of treatment with 0 and 10 μM of TCQA and 200 nM of α-MSH. The band intensities were done by comparing GAPDH using LI-COR system. (D) Gene expression of TYR, TYRP1, and DCT after 24 and 48 h with 0 and 10 μM of TCQA and 200 nM of α-MSH. The mRNA level was quantified using TaqMan real-time PCR. Results represent the mean ± SD of three independent experiments. *Statistically significant (P ≤ 0.05) difference between control and treated cells. **Statistically significant (P ≤ 0.01) difference between control and treated cells.