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. 2020 Mar 25;8:175. doi: 10.3389/fcell.2020.00175

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Copyright © 2020 Bejaoui, Villareal and Isoda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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3,4,5-Tri-O-caffeoylquinic acid (TCQA) enhanced the pigmentation in B16F10 murine melanoma cells. (A) Melanin content and cell viability determination after 48 and 72 h of treatment with various concentrations of TCQA and 200 nM of α-MSH used as positive control. (B) Determination of the protein expression of the melanogenesis enzymes – Tyr, Tyrp1, and Dct – after 24 and 48 h of treatment with 0 and 25 μM of TCQA and 200 nM of α-MSH. The band intensities were done by comparing GAPDH using LI-COR system. (C) Gene expression of Tyr, Tyrp1, and Dct after 24 and 48 h with 0 and 25 μM of TCQA and 200 nM of α-MSH. The mRNA level was quantified using TaqMan real-time PCR. Results represent the mean ± SD of three independent experiments. *Statistically significant (P ≤ 0.05) difference between control and treated cells. **Statistically significant (P ≤ 0.01) difference between control and treated cells.