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. 2020 Mar 25;10:244. doi: 10.3389/fonc.2020.00244

Figure 3.

Figure 3

Identification of MCF-7 and MDA-MB-231 cells apoptosis induced by the combination Ad-VT and paclitaxel using Hoechst staining and Annexin V assays. (A) Morphological changes visualized by fluorescence microscopy after Hoechst staining. MCF-7 and MDA-MB-231 cells were infected with Ad-VT, paclitaxel, and Ad-VT and paclitaxel mixture, stained with Hoechst stain at 72 h. Nuclear thickening and nuclear fragmentation increased significantly with time in Ad-VT and in the combination Ad-VT and paclitaxel groups. (B) MCF-7 and MDA-MB-231 cells apoptosis was analyzed by flow cytometry after Annexin-V FITC/PI staining. MCF-7 and MDA-MB-231 cells infected with Ad-VT and combination of Ad-VT and paclitaxel exhibit abundant apoptosis. All measurements were performed in triplicate. *p < 0.05, ***p < 0.001, ****p < 0.0001.