Figure 7.

Screening and identification of MDA-MB-231-LUC cells. (A) After transfection with the pGL4.51 plasmid, the two cell clones with the highest luciferase activity, were screened with 400 μg/ml of G418. (B) The luciferase activity of each cell clone was detected using a ONE-Glo™ Luciferase Assay System. The luciferase activity (RLU) of cell clones were detected every five generations using the luciferase assay kit to determine whether the Luc gene was stably expressed. (C,D) The cell clone with the highest luciferase activity was inoculated in a 1:2 ratio into a 96-well plate. After adding fluoresce in, the relationship between bioluminescence intensity and cell number was observed. The cell bioluminescence intensity increased with the increase of cell number and Clone 24 was found to stably express luciferase. (E) The MDA-MB-231 and MDA-MB-231-LUC cells were cultured in 96-well cell culture plates, and cell growth trends were detected at 1, 2, 3, 4, 5, 6, and 7 days. (F,G) The MDA-MB-231 and MDA-MB-231-LUC cells were cultured in 12-well cell culture plates, and cell cycles were detected using flow cytometry.