Table 3.
Primers used in this study for SNP analysis of B. anthracis.
| SNP group | Sequences (from 5′ end to 3′ end) | Probe sequences (from 5′ end to 3′ end) |
|---|---|---|
| A.Br.001 | CAAGCGGAACCAAATTTAATCTTT | FAM-ACCGAAACTTGAAGTC - MGB |
| TTCACCGTACGTCATTGTATAATACG | VIC-AAACCGAAATTTGAAGTC - MGB | |
| A.Br.002 | AACGATACCTAAAATCGATAAAG | FAM-CGCCCAGCCTAA-MGB |
| GGCAGAAGGAGCAAGTAATGTT | VIC-CGCCCAACCTAAA -MGB | |
| A.Br.003 | GCTACTGTCATTGTATAAAAACCTCCTTT | FAM-TACCTCAAGCTTAATTC- MGB |
| CGCTTGCCAAGCTTTTTTTC | VIC-CTACCTCAAACTTAATTC- MGB | |
| A.Br.004 | CCGATACCAGTAAACGACGACAT | FAM-TTGGAATGCCCCTAAT- MGB |
| CTGGAATTGGTGGAGCTATGGA | VIC-CTTTGGAATGTCCCTAAT- MGB | |
| A.Br.006 | CCGGAAATTGCTATTAGAACGAA | FAM-CCATACGCCTAGTGC-MGB |
| TCCCAATCTAGCGTTTTTAAGTTCA | VIC-CATCGCCTCGTGCA-MGB | |
| A.Br.007 | TTGGTAACGAGACGATAAACTGAATAA | FAM-CATCCTTACATTCAGCT-MGB |
| GCCTTGGATTGGCGATTG | VIC-CCATCCTTATATTCAGCTC -MGB | |
| A.Br.008 | TTCGCAACTACGCTATACGTTTTAGAT | FAM-ATAATTCTTCGCCGCTTG-MGB |
| CAAACGGTGAAAAAGTTACAAATATACG | VIC-ATTCTTCTCCGCTTGTT-MGB | |
| A.Br.009 | GGCAATCGGCCACTGTTT | FAM-CGGCTTTGCTTGC-MGB |
| GGGTTTCTACTGTGTATGTTGTTAATAAAAAG | VIC-CGGCTTTACTTGCATC-MGB | |
| B.Br.001 | TGCATGCTTCTTCTTACAGAGTAGTTAAT | FAM-CGATACCTTCTTATCCTC-MGB |
| CGGTCATAAAAGAAATCGGTACAA | VIC-CGATACCTTCTTATCTTC-MGB | |
| B.Br.002 | TGTTGCACCTTCTGTGTTCGTT | FAM-CGTTACTGCTGTTCC-MGB |
| GTAGTGGCTTCACCGAATGGA | VIC-AACGTTACTTCTGTTCCT-MGB | |
| B.Br.003 | CATTTATTCGCATAGAAGCAGATGA | FAM-ACATATCCACTTCACG- MGB |
| TGTGCCATCAAATAACTCTTTCTCAA | VIC-CATATCCGCTTCACG-MGB | |
| B.Br.004 | GAAGTTAAGTATCAACCAGCAGAAGAAA | FAM-CTTTACTTCTATCATCCC-MGB |
| CCGCCGCCTTGAGCTT | VIC-CTTTACTTCTACCATCCC-MGB | |
| A/B.Br.001 | GAAGGTCTCCAATTTGGATTTAAAAT | FAM-TTTTATTTAGAAGATAGCGGC-MGB |
| CGTGTGAACCTTTCGGTAAATAGTC | VIC-TTTATTTAGGAGATAGCGGC -MGB |
B. anthracis, Bacillus anthracis; SNP, single-nucleotide polymorphism.