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. 2020 Mar 25;13:49. doi: 10.3389/fnmol.2020.00049

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Copyright © 2020 di Caudo, Martínez-Valbuena, Mundiñano, Gennetier, Hernandez, Carmona-Abellan, Marcilla Garcia, Kremer and Luquin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Coronal sections of animals injected with canine adenovirus type (CAV)-GFP in the left putamen. (A) Low magnification of immunohistochemistry (IHC) against GFP and Nissl counterstained of the injected putamen; (B) higher magnification of IHC against GFP and Nissl counterstained of the injected thalamus; (C) IHC against GFP and Nissl counterstained of the substantia nigra (SN) of the injected hemisphere; (D) IHC against GFP (pink) and NeuN (black) in the SN of the injected hemisphere; and in the contralateral SN (E) immunofluorescence (IF) against GFP (green; F) IF against TH (red; G) merge of (E,F). White arrows denoted TH⁺/GFP⁺ cells. Scale bars: (A) 1 mm; (B,C) 100 μm; (D) 10 μm; (E) 5 μm.