Table 2.
Hematological safety analysis of 28 days repeated dosing of SKK in male Wistar rats: Parameters such as hemoglobin (Hb), total red blood corpuscles (RBC), hemoglobin per RBC (MCH), mean corpuscular hemoglobin concentration per unit volume (MCHC), mean corpuscular size (MCV), the total and differential counts of leukocytes were tested in the whole blood collected from the Wistar rats exposed to Divya Sarva-Kalp-Kwath (SKK) decoction up to the concentration of 1,000 mg/kg.
| Gr. no. | Dose (mg/kg) | Hb (g/dL) | Total RBC (×106/mm3) | RBC indices | Total WBC (×103/mm3) | Differential leucocyte count (%) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MCH (pg/cell) | MCV (fL/cell) | MCHC (g/dL) | Neutrophil | Lymphocyte | Eosinophil | Macrophage | Basophil | |||||
| 1 | 0 | 16 ± 1 | 7.8 | 21 ± 1 | 53 ± 3 | 39 | 16 ± 5 | 21 ± 8 | 73 ± 9 | 1 | 4 ± 2 | 1 |
| 2 | 100 | 16 | 7.7 | 21 ± 1 | 53 ± 2 | 39 ± 1 | 14 ± 6 | 22 ± 6 | 70 ± 6 | 2 | 4 ± 1 | 1 |
| 3 | 500 | 16 ± 1 | 7.5 | 10 ± 2 | 54 ± 1 | 39 ± 1 | 15 ± 5 | 24 ± 5 | 70 ± 5 | 1 | 3 ± 1 | 2 |
| 4 | 1,000 | 15 ± 4 | 7 ± 1 | 21 ± 1 | 53 ± 1 | 39 ± 1 | 15 ± 4 | 20 ± 5 | 72 ± 5 | 2 ± 2 | 5 ± 2 | 1 |
No changes were observed in the SKK treated animals in comparison to the normal control animals (0 mg/kg). The results indicated that SKK was safe up to five times the human equivalent oral dose given to the CCl4 treated Wistar rats in the pharmacological effect study.