Table 3.
Effect of 28-days oral exposure of SKK on blood biochemistry in wistar rats: Biochemical parameters such as urea, creatine, albumin, alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin, glucose, and cholesterol levels were determined in the serum of Wistar rats exposed to varying concentrations of the Divya Sarva-Kalp-Kwath (SKK).
| Gr. no. | Dose (mg/kg) | Urea (mg/dL) | Creatinine (mg/dL) | Albumin (g/dL) | ALT (U/L) | AST (U/L) | ALP (U/L) | Total Bilirubin (mg/dL) | Glucose (mg/dL) | Total Cholesterol (mg/dL) |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 0 | 53 ± 3 | 1 | 4 | 112 ± 15 | 288 ± 36 | 446 ± 100 | 0.04 ± 0.05 | 103 ± 5 | 63 ± 4 |
| 2 | 100 | 48 ± 4 | 0.6 | 3 | 109 ± 22 | 264 ± 24 | 464 ± 100 | 0.04 ± 0.09 | 101 ± 7 | 54 ± 6 |
| 3 | 500 | 51 ± 4 | 0.5 | 3 | 114 ± 18 | 224 ± 36 | 387 ± 79 | 0.04 ± 0.05 | 102 ± 8 | 56 ± 11 |
| 4 | 1,000 | 48 ± 7 | 0.5 | 3 | 115 ± 1 | 199 ± 47 | 439 ± 137 | 0.06 ± 0.09 | 100 ± 6 | 57 ± 10 |
Based on the analysis, no change in these serum-based biochemical parameters was observed in the animals exposed up to the dose of 1,000 mg/kg of SKK in comparison to the normal control animals (0 mg/kg). No changes were observed in the SKK treated animals in comparison to the normal control animals (0 mg/kg). The results indicated that SKK was safe up to five times the human equivalent oral dose given to the CCl4 treated Wistar rats in the pharmacological effect study.