FIGURE 3.

LPS induces intensive proinflammatory cytokines during PRRSV infection. PAMs were mock infected or infected with CHR6 (MOI = 1) in the presence or absence of LPS (100 ng/mL) for indicated time. Total RNA was extracted from cell lysates at 3, 6, and 12 hpi. The relative expression of IL-1β (A), IL-6 (B), IL-8 (C), and TNF-α (D) was analyzed using qRT-PCR. Data are normalized to HPRT1 in each individual sample. Data are the results of three independent experiments (means ± SE). Significant differences are denoted by *P < 0.05, **P < 0.01, and ***P < 0.001.