Figure 14.
CLSM images of MC3T3-E1 cell morphology on different samples after culturing for 1 and 3 days (A–G, a–g); the quantitative analysis of immunofluorescence intensity of vinculin is also shown (H).
Notes: Uppercase letters indicate the day 1 images, lowercase letters indicate the day 3 images: (A, a), 5VZn surface cultured with CM; (B, b), 15VZn surface cultured with CM; (C, c), 25VZn surface cultured with CM; (D, d), 5V surface cultured with CM; (E, e), 15V surface cultured with CM; (F, f), 25V surface cultured with CM; (G, g), Ti. (H) The relative immunofluorescence intensity of vinculin on different surfaces, compared to Ti. Data are expressed as the mean ± standard deviation (n=3). $Denotes the statistical significance between Ti and other groups. #Denotes the statistical significance between each Zn-incorporated TNT group and its TNT counterpart group. &Denotes the statistical significance between different labeled groups. ($P<0.05, #P<0.05, &P<0.05, ANOVA.)
Abbreviations: CLSM, confocal laser scanning microscope; CM, conditioned medium.