Figure 3.

Hepatocyte‐specific expression of human CES1 ameliorates Western diet–induced hyperlipidemia by inducing LDLR, ApoC‐II, and LPL. C57BL/6 mice were injected intravenously with AAV8‐ALB‐Null or AAV8‐ALB‐hCES1 and fed a Western diet for 16 weeks (n = 8 per group). Plasma levels of cholesterol (A), TG (B), and FFA were measured. (D) Plasma cholesterol lipoprotein profile was analyzed by FPLC. Hepatic mRNA levels were quantified by quantitative real‐time PCR (E). (F,G) Hepatic protein levels were determined by Western blotting. (H) Bile acid levels in the small intestine, gallbladder, or liver were measured, and total bile acid levels were determined. *P < 0.05, **P < 0.01. Abbreviations: GB, gallbladder; SI, small intestine.