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. 2020 Feb 6;4(4):606–626. doi: 10.1002/hep4.1483

Figure 7.

Figure 7

Fourteen genes were identified as the signature genes of alcohol‐activated hHSCs/mHSCs. (A) Fourteen genes with 2 or fewer connections within the network were identified as signature genes of alcohol‐activated hHSCs/mHSCs. The P value and Benjamini‐Hochberg–adjusted P value of alcohol‐activated (vs. normal) hHSCs are shown. (B) Functional enrichment analysis identified pathways associated with these 14 genes (six pathways are shown) and were grouped in a cross‐species protein–protein interaction network (https://https//string-db.org). The pathways shown are significantly enriched for these 14 genes with Benjamini‐Hochberg–adjusted P < 0.05. (C) Expression of selected genes was analyzed by quantitative real‐time PCR in normal hHSCs (n = 3, N5, N6, N7) and ALD hHSCs (n = 3, A3, A4, A5) isolated from a different set of donors (*P < 0.05, **P < 0.01, ***P < 0.001; Student t test and one‐way analysis of variance). (D) Donor information: N5‐N7, donors without underlying liver disease; A4‐A6, donors with ALD who were diagnosed based on liver function, histology, and history of alcohol abuse. Abbreviations: ALT, alanine aminotransferase; AST, aspartate aminotransferase. Abbreviations: CD14, CD14 molecule; CD53, CD53 molecule; CD74, CD74 molecule; CSF1R, Colony stimulating factor 1 receptor; CTSS, Cathepsin S; CYBA, Cytochrome b‐245 alpha chain; IRF7, Interferon regulatory factor 7; ITGB2, Integrin subunit beta 2; LAPTM5, Lysosomal protein transmembrane 5; MMP9, Matrix metallopeptidase 9; PLEK, Pleckstrin; SPP1, Secreted phosphoprotein 1; TREM2, Teneurin transmembrane protein 2; TYROBP, TYRO protein tyrosine kinase binding protein.