Fig. 4.

Schematic representation of the localization of wild-type and mutated BAP1 protein. BAP1 protein normally localizes itself in the cell nucleus due to the presence of the nuclear localization sequence (NLS). In the presence of an ubiquitin conjugating enzyme (UBE20), NLS will be covered with ubiquitin localizing the BAP1 protein in the cell's cytoplasm. Wild-type BAP1 protein has a capability to remove the ubuiquitin and hence is able to enter back into the nucleus. Mutated BAP1 protein loses its autodeubiquitination property hence it remains in the cytoplasm.