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. 2020 Mar 25;10:305. doi: 10.3389/fonc.2020.00305

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Copyright © 2020 Liu, Fan, Chen, Zhang and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CaSR effect on F-actin ring formation of OCs. (A,B) Four groups of OC precursor cells were treated with CM from modified A549 cells co-cultured with macrophages to examine potential OC differentiation induction. Immunofluorescent staining was used to evaluate F-actin ring formation of OCs. The average area of F-actin ring of OCs was detected and calculated using 400-fold microscope and ImageJ software (NIH). The average area of F-actin ring of OCs in CaSR overexpression group was significantly higher than that in empty vector and negative control groups (p < 0.001). The difference between negative and empty vector control groups was not statistically significant (p > 0.05). **p < 0.01, ***p < 0.001.