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. 2020 Mar 25;10:305. doi: 10.3389/fonc.2020.00305

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Copyright © 2020 Liu, Fan, Chen, Zhang and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CaSR effect on osteocalcin expression of OBs. (A,B) Four groups of OB precursor cells were treated with CM from co-cultured modified A549 cells with macrophages to examine potential OB differentiation induction. Immunofluorescent staining was used to detect expression of osteocalcin-positive OBs. The relative fluorescence intensity of osteocalcin of OBs in CaSR overexpression group was significantly lower than that in empty vector and negative control groups (p < 0.001). There was no significant difference between empty vector and negative control groups (p > 0.05). **p < 0.01, ***p < 0.001.