Figure 1.
Characterization of MHC Class I-Associated Immunopeptidome (MIP) Derived from the Visceral Adipose Tissue (VAT) of NCD-Fed (Lean) and HFD-Fed (Obese) Mice
(A)Schematic representation of the experimental workflow used to generate and identify VAT MIPs. The epididymal fat pad (VATs) from NCD and HFD-fed C57BL/6 mice were extracted. H2-Kb-associated peptides were isolated independently by immunoaffinity purification using the monoclonal antibodies Y-3, and the eluted peptides were identified using LC-MS/MS.
(B) The column diagram illustrating the length distribution of NCD- and HFD VAT MIPs (8–11 amino acids).
(C) The graphs indicating the distribution of the predicted affinity values of NCD- and HFD VAT MIPs by netMHCpan4.0.
(D) The binding motifs for octameric and nonameric peptides identified in NCD- and HFD VAT MIPs, respectively, were illustrated. The x-axis represents the residue position within octameric and nonameric peptide sequences. The y axis represents the information content, with the size of each amino acid symbol proportional to its frequency.