Figure 5.

Peripheral CD8⁺ T Cells Recognize LDHA_237-244 Peptide in HFD-Induced Obese Mice

(A) Tandem mass spectra of endogenous LDHA_237-244 (upper: m/z 461.76538Da) and its corresponding synthetic peptide (lower: m/z 461.76566Da).

(B) Statistical graph of the frequency of IFN-γ- and TNF-α-producing cells among the CD8⁺ T cells primed by various concentrations of LDHA_237-244 peptide.

(C) Exemplified FACS plots of the lymphocytes from the mesenteric lymph nodes of HFD-fed (obese) mice and NCD-fed (lean) mice stained with H2-Kb/LDHA_237-244 pentamer (Proimmune) and anti-mouse CD8 antibody.

(D) Summary graph of FACS analysis of the frequency of H2-Kb/LDHA_237-244 pentamer positive CD8⁺ T cells in the mesenteric lymph nodes from NCD and HFD-fed mice after 8-week HFD or NCD administration (n = 6 per group). The results are representative of one of two independent experiments. Data are presented as scatterplots with minimum and maximum values for data distribution. ∗∗p < 0.01.

See also Figure S4.