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. 2020 Mar 25;13(4):100747. doi: 10.1016/j.tranon.2020.100747

Figure 5.

Figure 5

Overexpression of WIF1 and JNK inhibition result in decreased prostate cancer cell invasion. (A) Western blot of the efficiency of WIF1 overexpression in 293T cells after the addition of CM, radioimmunoprecipitation buffer, or phosphate-buffered saline). (B) LNCaP cell invasion was significantly decreased after the addition of CM from WIF1-overexpressing 293T cells (P = .009). (C) The addition of shWNT5a-21 resulted in decreased JNK phosphorylation, as indicated by Western blot. (D) HS-5 CM with WNT5A shRNA resulted in decreased JNK phosphorylation, as indicated by Western blot. (E) LNCaP cells with HS-5 CM showed significantly decreased cell invasion in the presence of the JNK II inhibitor (P = .03). The presence of the JNK II inhibitor alone did not affect LNCaP cell invasion.