FIGURE 3.

Hypothalamic expression levels of weight-related molecules, neuropeptides, and their receptors (A), transcription factors (B), various peripheral signaling molecules (C), and growth factors (D) in animals from 5HT sublines, measured by RT-qPCR analysis. Expression levels were normalized to mean of two reference genes (Actb and Gapdh) and are shown as relative expression ratio between high-5HT and low-5HT subline. Mean ± SEM, n = 9–10 rats/subline. Abbreviations: Agrp, agouti-related peptide; Adipor, adiponectin receptor; Bdnf, brain-derived neurotrophic factor; Cart, cocaine-and amphetamine-related transcript; Cebpa, Cebpb Cebpd, CCAAT/enhancer binding protein alfa, beta, delta; Fgf, fibroblast growth factor; Glut3, glucose transporter 3; Hcrt, hypocretin; Hcrtr, hypocretin receptor; Insr, insulin receptor; Irs, insulin receptor substrate; Lepr, leptin receptor; Mch, melanin-concentrating hormone; Npy, neuropeptide Y; Npyr, neuropeptide Y receptor; Pomc, pro-opiomelanocortin; Ppargc1b, peroxisome proliferator-activated receptor gamma coactivator 1 beta; Stat3, signal transducer and activator of transcription 3.