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. 2019 Mar 6;10(2):124–131. doi: 10.1016/j.jtcme.2019.03.002

Fig. 1.

Fig. 1

High-performance liquid chromatography (HPLC) spectrum of RS and CA extracts

A: Standard HPLC chromatogram of flavonoids B. HPLC chromatogram of hydroalcoholic extract of RS leaves, C: HPLC chromatogram of aqueous extract of CA leaves, D: Standard HPLC chromatogram of sennosides, E: HPLC chromatogram of aqueous extract of CA leaves

Mobile system was consisting of methanol and 1.25% acetic acid in water at a flow rate of 1.0 ml/min at 40 °C, at λ max 270 nm for sennosides; Mobile system was consisting of acetonitril (45%) and 0.1% formic acid in water (55%) at a flow rate of 1.0 ml/min at 40 °C, at λ max 370 nm for the separation of flavonoids.