Table 1.
Primers used for quantitative polymerase chain reaction.
| Gene | Forward (5′-3′) | Reverse (5-3′) | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|---|
| PPARγ | ACCCAGAAAGCGATTCCTTCA | GTGTCAACCATGGTCATTTCTTGT | 60 | 70 |
| Ki67 | TGAGCCTGTACGGCTAAAACA | CACTCTCATCAGGGTCAGAAGA | 60 | 179 |
| ZO-1 | TTCTGAGGCCTGTAACCATTT | AATTGGATACCACTGGGCATAC | 60 | 245 |
| IL-6 | TTCGGTACATCCTCGACG | CAGGCAAGTCTCCTCATT | 60 | 167 |
| Caspase-3 | CATGGAAGCGAATCAATGGACT | CTGTACCAGACCGAGATGTCA | 60 | 139 |
| SREBP-1 | GGAGCCATGGATTGCACTTT | TCAAATAGGCCAGGGAAGTCA | 60 | 77 |
| FAS | GACCGCTTCCGAGATTCC | GATGGCAGTCAGGCTCAC | 60 | 137 |
| FADS-2 | TGTCTACAGAAAACCCAAGTGG | TGTGGAAGATGTTAGGCTTGG | 60 | 128 |
| GAPDH | TCGGAGTCAACGGATTTGGT | TTCCCGTTCTCAGCCTTGAC | 60 | 181 |