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. 2019 Nov 27;16:30–40. doi: 10.1016/j.omto.2019.11.001

Figure 3.

Figure 3

miR-489-3p/SIX1 Axis Regulates Glycolysis in Melanoma Cells

(A) A375 cells were transfected with miR-489-3p mimics or miR-489-3p mimics plus the SIX1 expression vector. Glucose uptake, lactate production, and ATP production were measured. Representative immunoblot reveals the expression of SIX1. qRT-PCR analysis indicates miR-489-3p expression. (B) A375 cells were transfected as in (A), and extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) were determined. (C) Lentivirus-mediated SIX1 knockdown (SIX1 shRNA) or control A375 cells were transfected with NC or miR-489-3p mimics. Glucose uptake, lactate production, and ATP production were measured and analyzed. Representative immunoblot reveals the expression of SIX1. (D) A375 cells were transfected as in (C). ECAR and OCR were determined. All values shown are the average values with error bars representing the SE (*p < 0.05, **p < 0.01 versus corresponding control). Each experiment was carried out in triplicate, repeated at least twice, and one representative result is given (A–D).