Figure 4.

miR-489-3p/SIX1 Axis Regulates Glycolysis, Tumor Growth, and Metastasis In Vivo

(A) A375 cells stably infected with lentivirus carrying the indicated constructs were injected into nude mice as indicated. Roman numerals listed are mice. (B) At the indicated times, the tumors were measured (mean ± SE; n = 7), and the growth curves were plotted. **p < 0.01 at day 45. (C) Lactate production of representative tumor tissues from (A). All values shown are the average values of quintuplicate measurements with error bars representing the SE. **p < 0.01 versus empty vector. (D) Immunoblot analysis of the expressions of SIX1 and the EMT markers (Slug and Vimentin) in representative excised tumor from (A). (E) Melanoma cell metastases were established in nude mice (n = 5) by tail vein injection of A375 cells expressing indicated constructs. Bioluminescence images were collected at 30 days. Each experiment was repeated at least twice, and one representative result is given (A–E).