Figure 5.

Correlation between miR-489-3p and SIX1 and Correlation of miR-489-3p with Glucose Uptake in Human Melanoma Patients

(A) Representative IHC of 39 melanoma patients. SIX1 and S100 were determined by IHC and miR-489-3p by MISH. (B) The correlation of miR-489-3p with SIX1 in melanoma patients from (A) was analyzed. The low, medium, and high expression of SIX1 was determined as described in the Materials and Methods. Horizontal lines inside the box represent the median; the bottom and top of the boxes represent the 25th and 75th percentiles. The lines above and below the box represent the upper and lower extremes. The vertical bars represent the range of data. Data were analyzed by one-way ANOVA with Games-Howell correction. (C) Analysis of correlation of miR-489-3p expression with SIX1 mRNA using the data from The Cancer Genome Atlas (TCGA) (https://cancergenome.nih.gov/). (D) Representative FDG PET scans of two representative cases (case 1, melanoma with metastasis; case 2, melanoma with no metastasis) and IHC or miRNA in situ hybridization (MISH) of 18 melanoma patients. SIX1 and S100 were examined by IHC and miR-489-3p by MISH. Arrows reveal primary tumor glucose uptake. Red circle indicates the metastasis uptake. Scale bars, 100 μm. The correlation of glucose uptake with SIX1 or miR-489-3p expression was determined by the Mann-Whitney U test. (E) Proposed model for miR-489-3p modulation of SIX1 expression and subsequent regulation glycolysis-related tumor growth and metastasis.