REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit Anti-Human SAMHD1 Polyclonal Antibody	Proteintech	Cat# 12586-1-AP, RRID:AB_2183496
Mouse Anti-Actin, beta Monoclonal Antibody	Abcam	Cat# ab6276, RRID:AB_2223210
Rabbit polyclonal CDK1 Antibody	Bethyl	Cat# A303-664A, RRID:AB_11204758
Mouse Anti-BM28 Monoclonal Antibody	BD Biosciences	Cat# 610701, RRID:AB_398024
Phospho-SAMHD1 (Thr592) (D7O2M) Rabbit mAb antibody	Cell Signaling Technology	Cat# 89930, RRID:AB_2800147
Mouse Anti-P21 Monoclonal antibody, Unconjugated, Clone f-5	Santa Cruz Biotechnology	Cat# sc-6246, RRID:AB_628073
IRF-3 (D6I4C) XP® antibody	Cell Signaling Technology	Cat# 11904, RRID:AB_2722521
NFkappaB p65 (F-6) antibody	Santa Cruz Biotechnology	Cat# sc-8008, RRID:AB_628017
Purified anti-H2A.X Phospho (Ser139) antibody	BioLegend	Cat# 613402, RRID:AB_315795
53BP1 antibody	BD Biosciences	Cat# 612522, RRID:AB_2206766
Anti-IFNα/β Receptor	PBL Interferon Source	N/A
Mouse IgG2A Isotype Control (Clone 20102) antibody	R and D Systems	Cat# MAB003, RRID:AB_357345
Rat Anti-IL-6 Monoclonal Antibody, Unconjugated, Clone MQ2-13A5	BD Biosciences	Cat# 554543, RRID:AB_398568
Mouse Anti-IL-8 Monoclonal Antibody, Unconjugated, Clone G265-5	BD Biosciences	Cat# 554716, RRID:AB_395526
Mouse Anti-TNF Monoclonal Antibody, Unconjugated, Clone MAb1	BD Biosciences	Cat# 551220, RRID:AB_394098
Rabbit Anti-Human TICAM1 Polyclonal Antibody, Unconjugated	GeneTex	Cat# GTX104744, RRID:AB_1241389
TBK1 Antibody	Bethyl	Cat# A300-093A, RRID:AB_2303002
Bacterial and Virus Strains
VSV-G HIV-1 GFP virus	Besnier et al., 2002	N/A
SIVmac Virus like particles	Goujon et al., 2008, Reinhard et al., 2014	N/A
Biological Samples
PBMC from HIV seronegative donors	This study	N/A
Chemicals, Peptides, and Recombinant Proteins
AB Human Serum	Sigma	#H4522
FBS	Biosera	N/A
BX795	APEXBIO	#A8222
Ruxolitinib	Cell guidance system	#SM87
Recombinant human IFN-b	Peprotech	#300-02BC
Recombinant human Tenascin C	R and D	#3358-TC-050
Polymyxin B	Sigma	#P4932
LPS	InvivoGen	#tlrl-prslps
TAK242	Milllipore	#614316
Recombinant Flagellin protein	Abcam	#Ab201366
DMEM, high glucose, pyruvate	Invitrogen	#41966052
RPMI 1640 medium	Invitrogen	#21875091
Opti-MEM	Invitrogen	#31985047
Fugene HD transfection reagent	Promega	#E2311
DAPI	Sigma	#10236276001
PhosSTOP	Sigma	#4906845001
Fast SYRB green master mix	ThermoFisher	#4385610
Total RNA Purification Kit	Norgen	#17200
Critical Commercial Assays
CellRox	Molecular Probes	#C10444
NuPAGE 4-12% Bis-Tris Protein Gel	Invitrogen	#NP0322BOX
pHrodo E.coli BioParticles	ThermoFisher	#P35361
SuperScript III First-Strand Synthesis System	ThermoFisher	#18080051
Amersham ECL Prime Western Blotting Detection Reagent	GE Healthcare	#RPN2232
Experimental Models: Cell Lines
293T	Laboratory of G. Towers (UCL)	N/A
Oligonucleotides
CDK1 FWD 5′TGAGGAACGGGGTCCTCTAA 3′	Invitrogen	N/A
CDK1 R 5′ A TGGCT ACCACTTGA CCTGT 3′	Invitrogen	N/A
CDK2 F 5′ AAGTTGACGGGAGAG GTGGT 3′	Invitrogen	N/A
CDK2 R 5′ TGATGAGGGGAAGAG GAATG 3′	Invitrogen	N/A
CDK4 F 5′ CAGATGGCACTTACACCCGT 3′	Invitrogen	N/A
CDK4 R 5′ CAGCCCAATCAGGTC AAAGA 3′	Invitrogen	N/A
CDK6 F 5′ CGTGGTCAGGTTGTT TGATGT 3′	Invitrogen	N/A
CDK6 R 5′ CGGTGTGAATGAAGAA AGTCC 3′	Invitrogen	N/A
Cyclin A2 F 5′ AAGACGAGACGGGTTGC 3′	Invitrogen	N/A
Cyclin A2 R 5′ GGCTGTTTACTGTTTGCTTTCC 3′	Invitrogen	N/A
Cyclin B1 F 5′ TTCTGGATAATGGTGAATGGAC 3′	Invitrogen	N/A
Cyclin B1 R 5′ ATGTGGCATACTTGTTCTTGAC 3′	Invitrogen	N/A
Cyclin D1 F 5′ AGATGAAGGAGACCATCCCCC 3′	Invitrogen	N/A
Cyclin D1 R 5′ CCACTTGAGCTTGTTCACCA 3′	Invitrogen	N/A
Cyclin D3 F 5′ GGCCGGGGACCGAAACT 3′	Invitrogen	N/A
Cyclin D3 R 5′CAGTGGCGAAGTGTTTACAAAGT 3′	Invitrogen	N/A
Cyclin E1 F 5′ CCGGTATATGGCGACACAAG 3′	Invitrogen	N/A
Cyclin E1 R 5′ TACGCAAACTGGTGCAACTT 3′	Invitrogen	N/A
Cyclin E2 F 5′ TCTCCTGGCTAAATCTCTTTCTCC 3′	Invitrogen	N/A
Cyclin E2 R 5′ ACTGTCCCACTCCAAACCTG 3′	Invitrogen	N/A
E2F1 F 5′ TGCCAAGAAGTCCAAGA ACCA 3′	Invitrogen	N/A
E2F1 R 5′ GTCAACCCCTCAAGCCGTC 3′	Invitrogen	N/A
E2F4 F 5′ CGGACCCAACCCTTCT ACCT 3′	Invitrogen	N/A
E2F4 R 5′ GGGGCAAACACTTCTGAGGA 3′	Invitrogen	N/A
E2F7 F 5′ CCTTTAGCCCACCCAGTATTT 3′	Invitrogen	N/A
E2F7 R 5′ A TCCCTCTCTGACCCTGACC 3′	Invitrogen	N/A
MCM2 F 5′CACCCGAAGCTCAACCA GAT 3′	Invitrogen	N/A
MCM2 R 5′ATCATGGACTCGATGTG CCG 3′	Invitrogen	N/A
RB1 F 5′AAAGGACCGAGAAGGACCA 3′	Invitrogen	N/A
RB1 R 5′AAGGCTGAGGTTGCTTGTGT 3′	Invitrogen	N/A
WIF1 F 5′TCTGTTCAAAGCCTGTCTGC 3′	Invitrogen	N/A
WIF1 R 5′ACATTGGCATTTGTTGGGTT 3′	Invitrogen	N/A
p14 F 5′GAGTGAGGGTTTTCGTGGTTC 3′	Invitrogen	N/A
p14 R 5′ACGGGTCGGGTGAGAGTG 3′	Invitrogen	N/A
p16 F 5′ CGGCTGACTGGCTGGC 3′	Invitrogen	N/A
p16 R 5′ GGGTCGGGTGAGAGTGG3′	Invitrogen	N/A
p21 F 5′GCCGAAGTCAGTTCCTTGTG 3′	Invitrogen	N/A
p21 R 5′TCGAAGTTCCATCGCTCACG 3′	Invitrogen	N/A
p27 F 5′A TGTTTCAGACGGTTCCCCA 3′	Invitrogen	N/A
p27 R 5′TCCAACGCTTTTAGAGGCAG 3′	Invitrogen	N/A
p53 F 5′AAGTCTAGAGCCACCGTCCA 3′	Invitrogen	N/A
p53 R 5′TTTCAGGAAGTAGTTTCCATAGGT 3′	Invitrogen	N/A
CXCL10 F 5′ TGGCATTCAAGGAGTACC TC 3′	Sigma	N/A
CXCL10 R 5′ TTGTAGCAATGATCTCAACACG 3′	Sigma	N/A
ISG56 F 5′CCT CCT TGG GTT CGT CTA CA 3′	Sigma	N/A
ISG56 R 5′GGC TGA TAT CTG GGT GCC TA 3′	Sigma	N/A
ISG54 F 5′CAGCTGAGAATTGCACTGC AA 3′	Sigma	N/A
ISG54 R 5′CGT AGGCTGCTCTCCAAG GA 3′	Sigma	N/A
MxA F 5′ATC CTG GGA TTT TGG GGC TT 3′	Sigma	N/A
MxA R 5′CCG CTT GTC GCT GGT GTC G 3′	Sigma	N/A
SAMHD1 F 5′TTGTGCTAGAGATAAGGAAGTTGG 3′	Invitrogen	N/A
SAMHD1 R 5′TGTGTTGATAAGCTCTACGGTG 3′	Invitrogen	N/A
GAPDH F 5′ACC CAG AAG ACT GTG GAT GG 3′	Sigma	N/A
GAPDH R 5′TTC TAG ACG GCA GGT CAG GT 3′	Sigma	N/A
ON-TARGETplus Human SAMHD1 siRNA	Dharmacon	#L-013950-01
Control siRNA	Santa Cruz	#sc-37007
TBK1 siRNA	Santa Cruz	#sc-39058
TICAM1 siRNA	OriGene	#SR315629
Recombinant DNA
GFP-encoding genome CSGW	Laboratory of G. Towers	N/A
p8.91	Laboratory of G. Towers	N/A
pMDG	Laboratory of G. Towers	N/A
SIVmac packaging plasmid encoding accessory genes	Laboratory of G. Towers	N/A
pCDNA Vpx E16A	Laboratory of J. Luban, Reinhard et al., 2014	N/A
Software and Algorithms
ImageJ		https://imagej.nih.gov/ij/
Other
Amersham Hybond P 0.45 PVDF blotting membrane	GE Healthcare	#10600023