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. 2020 Mar 24;30(12):4292–4302.e7. doi: 10.1016/j.celrep.2020.03.024

Figure 4.

Figure 4

Smoc1 Is Repressed by BMP Signaling

(A–C) BRE:GFP signal (green) and Smoc1 immunostaining (red) in the fin, at 48 hpf.

(D–G) Smoc1 expression pattern at 2 h post-heat-shock induction (hpHS) of a dominant-negative BMP receptor transgene (dnBmpr-GFP+) or in sibling controls (for heat-shock conditions, see STAR Methods), at 48 hpf. Inset, GFP immunostaining to monitor expression of transgene.

(H) Average intensities of normalized Smoc1 immunostainings and BRE:GFP signal versus relative position (ROI midline), at 48 hpf. Intensities were normalized to respective maxima.

(I) Average intensity of normalized Smoc1 immunostainings versus relative position (ROI midline) in dnBmpr-GFP+ transgenics and siblings control. Smoc1 intensity in dnBmpr-GFP+ was normalized to the control maxima. Shadowing corresponds to SEM per relative position (H, I).

Scale bars, 50 μm. n represents number of fins analyzed. Anterior, left; distal, down. BRE:GFP transgene used: BRE:eGFP (Laux et al., 2011) (A–C, H). See also Figure S3.