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. 2019 Jun 1;12(2):113–124. doi: 10.1093/jmcb/mjz045

Figure 2.

Figure 2

Mapping the interaction domains in p53 and RNF20/40. (A and B) The third coiled-coil regions of RNF20 or RNF40 mediated the interactions with p53. The internal deletion mutants of HA-tagged RNF20 or RNF40 and SFB-p53 were expressed in 293T cells. Cell lysates were examined by co-IP and western blots with the indicated antibodies. (C) The CTD of p53 is required for the interaction with RNF20/40. HA-tagged p53 internal deletion mutants and SFB-RNF 20 or SFB-RNF40 were expressed in 293T cells. The interaction between RNF20/40 and p53 were examined by co-IP and western blots with the indicated antibodies.