Figure 1.

Decreased H3K27me3 levels are associated with poor prognosis and chemoresistance in vivo. (A) Kaplan–Meier plot of overall survival of patients based on H2K27me3 levels. A log-rank test was used for statistical analysis. Scale bar, 100 μm. MFS, metastasis-free survival. (B) Immunoblotting analysis of H3K27me3 in six PDX tumors. (C) Immunohistochemical analysis of H3K27me3 levels in two PDX samples. (D–F) PDX#4 and #6 tumors were subcutaneously injected into the nude mice. The mice were treated with oxaliplatin when the tumor volume reached 50 ± 10 mm³. Oxaliplatin was administered by i.p. injection (1 mg/kg) every 4 days for 28 days. Tumor growth curves (D), images of isolated tumors (E), and tumor weights (F) of each indicated group are shown (n = 6).