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Identification of LSP-1 as the Target of VHH D4
In vitro-generated mo-DC or mo-Mac were incubated with VHH D4, then lysed. Immuno-precipitation was performed on cell lysate using the streptavidin-binding peptide tag. MW, molecular weight. Immuno-precipitated material was analyzed by western blot. Staining was performed using streptavidin-HRP (A) or a commercial anti-LSP-1 antibody (B).
